Journal: Molecular Cell

Article Title: The ribotoxic stress response drives acute inflammation, cell death, and epidermal thickening in UV-irradiated skin in vivo

doi: 10.1016/j.molcel.2024.10.044

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti-γ-tubulin , Merck , Cat#T5326; RRID: AB_532292.

Techniques: Recombinant, Reverse Transcription, In Situ, Knock-Out, Variant Assay, Software, Purification, Blocking Assay

Journal: Stem Cell Reports

Article Title: A feedforward loop between JAK/STAT downstream target p115 and STAT in germline stem cells

doi: 10.1016/j.stemcr.2023.08.007

Figure Lengend Snippet: p115 is a putative downstream target of JAK/STAT signaling in Drosophila testis (A) Dam-ID analysis for Stat92E reveals binding peaks of Stat92 E at the p115 region (orange dashed boxes). (B) qRT-PCR quantification of p115 mRNA levels in testes with indicated genotypes. Mean ± SD is shown (n ≥ 4). Two-tailed Student’s t test was used. ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001. (C) Western blot analysis of p115 protein levels in the adult testes with indicated genotypes. β-Tubulin was used as blotting control. (D) Quantification of p115 protein levels in testes with indicated genotypes by western blot analysis. Blot band intensity ratio of p115/β-tubulin shows similar trend with qRT-PCR in (B). Data are shown as mean ± SD (n = 3). Ordinary one-way ANOVA test was used. ∗ p < 0.05, ∗∗ p < 0.01.

Article Snippet: Goodman, DSHB), rabbit anti-GFP (1:1,000, Abcam), mouse anti-γ-tubulin (1:1,000, Abbkine), guinea pig anti-Stat92E (1:1,000, a generous gift from Dr. Yu Cai), mouse anti-pStat92E (1:2,000, Abmart, China; ), rabbit anti-β-galactosidase (lacZ, 1:5,000, Cappel, cat. no. 55978), and mouse anti-Flag (1:2,000, Sigma-Aldrich).

Techniques: Binding Assay, Quantitative RT-PCR, Two Tailed Test, Western Blot

Journal: Stem Cell Reports

Article Title: A feedforward loop between JAK/STAT downstream target p115 and STAT in germline stem cells

doi: 10.1016/j.stemcr.2023.08.007

Figure Lengend Snippet: p115 depletion affects spindle orientation (A and B) Representative images of division angles of the mitotic spindles (by γ-tubulin in yellow) in control (A) and nos > p115 RNAi GSCs (B). (C) Radial histogram quantification of division angles in control (n = 36) and p115 depleted (n = 62) metaphase GSCs at 1 day after eclosion. γ-Tubulin (yellow) is stained for the centrosome and DAPI (blue) is stained for the nucleus. Scale bars, 5 μm.

Article Snippet: Goodman, DSHB), rabbit anti-GFP (1:1,000, Abcam), mouse anti-γ-tubulin (1:1,000, Abbkine), guinea pig anti-Stat92E (1:1,000, a generous gift from Dr. Yu Cai), mouse anti-pStat92E (1:2,000, Abmart, China; ), rabbit anti-β-galactosidase (lacZ, 1:5,000, Cappel, cat. no. 55978), and mouse anti-Flag (1:2,000, Sigma-Aldrich).

Techniques: Staining

Journal: Stem Cell Reports

Article Title: A feedforward loop between JAK/STAT downstream target p115 and STAT in germline stem cells

doi: 10.1016/j.stemcr.2023.08.007

Figure Lengend Snippet: The levels of pStat92E and Stat92E are diminished in p115 -depleted GSCs (A) The levels of pStat92E (red) and Stat92E (green, by Stat92E-Flag-GFP) in control GSCs. GSCs are indicated by white dotted circles. The hub is indicated by asterisks. pStat92E and Stat92E channels are shown separately. (B) The levels of pStat92E (red) and Stat92E (green, by Stat92E-Flag-GFP) are greatly diminished in nos > p115 RNAi GSCs. (C) Quantification of the average fluorescent intensity of pStat92E and Stat92E in control and nos > p115 RNAi GSCs. Mean ± SD is shown (n = 20). Two-tailed Student’s t test was used. ∗∗∗∗ p < 0.0001. (D) Western blot analysis and quantification of the levels of pStat92E protein in adult testes with indicated genotypes. β-Tubulin was used as blotting control. Data are shown as mean ± SD (n = 3). Two-tailed Student’s t test was used. ∗ p < 0.05. (E–I) Representative confocal images of pStat92E (yellow) staining in adult testes of control and nos > p115 RNAi at different time points after eclosion. The levels of pStat92E gradually reduced over time in p115 -depleted GSCs (white arrowheads). The GBs with high pStat92E levels are indicated by yellow arrowheads. The hub is indicated by yellow asterisks. pStat92E channels are shown separately. (J) Quantification of the average fluorescent intensity of pStat92E in testes with indicated genotypes at different time points after eclosion. Mean ± SD is shown (n ≥ 45). Ordinary one-way ANOVA test was used. ∗∗∗∗ p < 0.0001. (K–O) Representative confocal images of Stat92E staining (red) in adult testes of control and nos > p115 RNAi at different time points after eclosion. The levels of Stat92E gradually reduced over time in p115 -depleted GSCs (white arrowheads). The hub is stained by FasIII in yellow. Stat92E channels are shown separately. (P) Quantification of the average fluorescent intensity of Stat92E in testes with indicated genotypes ate different time points after eclosion. Mean ± SD is shown (n ≥ 55). Ordinary one-way ANOVA test was used. ∗∗∗∗ p < 0.0001. Scale bars, 5 μm.

Article Snippet: Goodman, DSHB), rabbit anti-GFP (1:1,000, Abcam), mouse anti-γ-tubulin (1:1,000, Abbkine), guinea pig anti-Stat92E (1:1,000, a generous gift from Dr. Yu Cai), mouse anti-pStat92E (1:2,000, Abmart, China; ), rabbit anti-β-galactosidase (lacZ, 1:5,000, Cappel, cat. no. 55978), and mouse anti-Flag (1:2,000, Sigma-Aldrich).

Techniques: Two Tailed Test, Western Blot, Staining

Journal: Frontiers in Immunology

Article Title: CD4 + T Cell Fate Decisions Are Stochastic, Precede Cell Division, Depend on GITR Co-Stimulation, and Are Associated With Uropodium Development

doi: 10.3389/fimmu.2018.01381

Figure Lengend Snippet: Fluorescent reagents for imaging flow cytometry.

Article Snippet: Ch2 , Fix/Perm , Mouse anti-gamma tubulin-AlexaFluor488 (clone TU-30: EXBIO A4-465-C100).

Techniques: Imaging, Cytometry, Staining